mouse anti stx1  (Santa Cruz Biotechnology)

Journal: Cell reports

Article Title: Nanoscopic dopamine transporter distribution and conformation are inversely regulated by excitatory drive and D2 autoreceptor activity

doi: 10.1016/j.celrep.2022.111431

Figure Lengend Snippet:

Article Snippet: Mouse Anti-STX1 , Synaptic Systems , 110 011 RRID:AB_887844.

Techniques: Virus, Recombinant, Synthesized, Generated, Software

Journal: Journal of Lipid Research

Article Title: Membrane assembly of Shiga toxin glycosphingolipid receptors and toxin refractiveness of MDCK II epithelial cells [S]

doi: 10.1194/jlr.M083048

Figure Lengend Snippet: TLC overlay assay detection of neutral GSLs from MDCK II cells with antibodies (A) and identification of Stx GSL receptors using Stx1a, Stx2a, and Stx2e subtypes (B). A: GSL amounts applied for Gb3Cer and Gb4Cer detection correspond to 1 × 105 cells, and those for Lc2Cer and Forssman GSL detection are equivalent to 1 × 106 and 5 × 103 cells, respectively. B: GSL amounts employed for the Stx1a, Stx2a, and Stx2e TLC overlay assays correspond to 1 × 106 cells, respectively. GSLs were separated in solvent 1 for neutral GSLs (A, B) and bound primary anti-GSL antibodies as well as anti-Stx1 or anti-Stx2 antibodies (B) were visualized with AP-conjugated secondary antibodies and BCIP as the substrate.

Article Snippet: After one more washing step with PBS, the Stx1a-exposed slides were incubated with monoclonal mouse IgG anti-Stx1 antibody (clone VT109/4-E9B) and those exposed to Stx2a and Stx2e with monoclonal mouse IgG anti-Stx2 antibody (clone VT135/6-B9, both from Sifin GmbH, Berlin, Germany) in 1:500 dilution with 1% BSA at 4°C overnight.

Techniques: Overlay Assay, Solvent

Journal: Journal of Lipid Research

Article Title: Membrane assembly of Shiga toxin glycosphingolipid receptors and toxin refractiveness of MDCK II epithelial cells [S]

doi: 10.1194/jlr.M083048

Figure Lengend Snippet: Immunofluorescence microscopic detection of Stx-binding GSLs in MDCK II (A) and Vero-B4 cells (B). The epithelial cell lines were grown in chamber slides and incubated with Stx1a, Stx2a, or Stx2e as indicated. Bound Stx1a and Stx2 subtypes were detected with anti-Stx1 and anti-Stx2 antibody, respectively, in conjunction with Alexa Fluor 488®-labeled secondary antibodies. Fluorescence imaging was performed for the apical cell surface (apical plane), on intracellular level (midplane) and at the basal cell surface (basal plane). The immunofluorescence micrographs merged with the DAPI stains of the nuclear DNA are shown.

Article Snippet: After one more washing step with PBS, the Stx1a-exposed slides were incubated with monoclonal mouse IgG anti-Stx1 antibody (clone VT109/4-E9B) and those exposed to Stx2a and Stx2e with monoclonal mouse IgG anti-Stx2 antibody (clone VT135/6-B9, both from Sifin GmbH, Berlin, Germany) in 1:500 dilution with 1% BSA at 4°C overnight.

Techniques: Immunofluorescence, Binding Assay, Incubation, Labeling, Fluorescence, Imaging